| Abstract Detail
Breeding and Genetics Nunziata, Angelina [1], Ruggieri, Valentino [1], Frusciante, Luigi [1], Barone, Amalia [1]. Allele mining at the locus Gro 1 in Solanum wild species. THE gene (Gro1) responsible for resistance to Globodera rostochiensis race Ro1 is present in a Solanum tuberosum-Solanum spegazzini diploid hybrid (P40) and was localized on short arm of chromosome 7 (Barone et al., 1990). Paal et al. (2004) have definitively cloned and sequenced 10 different genes in a cluster at locus Gro 1, among which only the Gro 1-4 gene has been demonstrated to confer resistance.
Using sequence information available at EMBL/GenBank/DDBJ databases (accession numbers AY196151-AY196163) we have designed three primer pairs. The first was designed in a highly conserved region and allowed us to demonstrate the presence of at least one member of the gene cluster in 19 wild species analysed. The second primer pair was designed on a Gro 1-4 specific region and allowed us to demonstrate that no gene identical to Gro 1-4 was present in any wild potato species analysed.
Finally, we amplified and sequenced the major part of the LRR coding sequence of Gro1 gene in 16 wild species. 405 SNPs were found, varying from 11 SNPs in S. demissum to 36 in S. stoloniferum, S. jamesii and S. fendleri . The alignment of translated sequences was compared to the consensus sequence obtained from multialignment of Gro1 genes from GenBank. Polymorphisms were found and classified regarding to the position of amino acid substitutions and to the nature of amino acids involved.
The non synonymous/synonymous ratio (Ka/Ks) of the major part of the species was significatively different from 1, demonstrating that the gene is under selective pressure. Substitution of L amino acids were 80% conservative, while substitution of amino acids exposed to solvents were 45% non conservative.
References
Barone et al. 1990 Mol. Gen. Genet. 224, 177-182
Paal et al. 2004 Plant J., v. 38, no. 2, p. 285-297
Log in to add this item to your schedule
1 - University of Naples Federico II, Dept Soil, Plant and Environmental Sciences, Via Università 133, Portici (NA), Italy, 80055
Keywords:
Disease resistance
wild potato species
allele mining.
Session: Poster-31
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:199 |
