| Abstract Detail
Conference Wide Sharma, Rameshwar [1]. Molecular and Developmental analysis of tomato mutants defective in ethylene biosynthesis/action. FRUIT ripening in tomato is intimately linked to climacteric production of ethylene gas at the breaker stage of the fruits. Mutants defective in perception as well as biosynthesis of ethylene would be valuable assets to examine the role of this gaseous plant hormone in regulation of fruit ripening. Despite of the tomato being a model system, to study the molecular basis of fruit ripening, all known fruit ripening mutants are the one that have been found naturally occurring in the field. We have isolated EMS-induced tomato mutants with defects in ethylene perception and biosynthesis. These mutants are likely to be useful for the understanding the dynamics of ethylene regulated fruit ripening. Among the several mutants isolated, we have characterized in details two mutant viz., acetylene resistant (atr-1) and kinetin insensitive (kin-27). We have putatively characterized kin-27 as an ethylene underproducer and atr-1 as ethylene insensitive. Genetic segregation analysis of atr-1 and kin-27 has indicated the monogenic recessive nature of the mutations. The atr-1 mutant had more flowers per inflorescence compared to wild type. There was significant delay in ripening of fruits in atr-1and kin-27 mutants both on vine and also during post-harvest storage after picking at breaker stage. The relative transcript level of the ethylene receptor genes were similar during fruit ripening in both wild type and atr-1 mutant, with exception of LeETR2 expression during breaker stage of fruit ripening. The analysis of these mutants provides further insight on ethylene regulated ripening of fruits in tomato.
Log in to add this item to your schedule
1 - University of Hyderabad, School of Life Sciences, Hyderabad, 500 046, India
Keywords:
tomato
ripening
mutant
ethylene
tomato fruit.
Session: Poster-111
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:198 |
