| Abstract Detail
Breeding and Genetics Kelley, Kevin [1], Gillen, Anne [2], Whitworth, Jonathan [1], Novy, Richard [1]. Development of Molecular Markers for Introgression of Potato Leafroll Virus Resistance From S. etuberosum into Cultivated Potato. POTATO leafroll virus (PLRV) is a major virus pathogen of potato. Infection of potato can result in substantial reductions in yield as well as tuber quality via the expression of net necrosis. Solanum etuberosum, a wild relative of cultivated potato, is highly resistant to PLRV as well as other potato diseases and insect pests. Reproductively isolated from cultivated potato, S. etuberosum, was somatically hybridized with a S. tuberosum haploid x S. berthaultii hybrid. Successive backcrossing of the somatic hybrid to cultivated potato has produced progeny with improved agronomics and PLRV resistance from S. etuberosum. Segregation of PLRV resistance in a BC3 population suggests a monogenic trait. Using RFLP and CAPS markers, previously mapped in potato and tomato, PLRV resistance was localized to chromosome 4 in the segregating BC3 population. Having localized the genomic region associated with PLRV resistance, we report on our efforts in the identification of PCR-based molecular markers that may be used for marker-assisted selection for PLRV resistance.
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1 - United States Department of Agriculture, Agricultural Research Service, 1693 South 2700 West, Aberdeen, Idaho, 83210, United States
2 - United States Department of Agriculture, Agricultural Research Service, 3793 North 3600 East, Kimberly, Idaho, 83341, United States
Keywords:
Solanum etuberosum
Potato leafroll virus
Marker-Assisted Selection.
Session: PAA09-8
Location: Hall of Ideas Room G/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 3:45 PM
Abstract ID:191 |
