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Abstract Detail


Breeding and Genetics

Baldwin, Samantha [1], Genet, Russell [1], Macknight, Richard [2], Dodds, Ken [3], Jacobs, Jeanne M E [1].

Genetics of resistance to powdery scab (Spongospora subterranea) of potato.

POWDERY scab, caused by the protozoan pathogen Spongospora subterranea f. sp. subterranea is a disease of potatoes that is becoming increasingly important. The use of resistant potato cultivars is the most important factor in disease control. The breeding programme at Crop & Food Research has produced resistant potato cultivars and breeding for resistance continues to be a major objective. However, this is a time-consuming process because disease expression is greatly affected by environmental influences and requires field screening to be repeated over several seasons. The potato breeding process would benefit greatly from the use of marker-assisted selection (MAS) for this trait. To search for markers associated with resistance an F1 mapping population of 450 tetraploid lines was produced by crossing a susceptible cultivar and a resistant breeding line. Currently three glasshouse screens and two field screens have been used to assess the resistance to powdery scab of 314 lines, 94 of which have been replicated over multiple tests. Molecular marker analyses including SCARs, SSRs, AFLPs and CAPS are being carried out on the mapping population. ANOVA analysis is being used to detect those markers with significant associations with disease resistance. Markers associated with disease resistance will be tested on a germplasm collection to assess their efficiency of predicting resistance and their possible application in a marker-assisted breeding programme.


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1 - New Zealand Institute for Crop & Food Research Ltd, Private Bag 4704, Christchurch, , New Zealand
2 - Otago University, Department of Biochemistry, Dunedin, , New Zealand
3 - AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, , New Zealand

Keywords:
MAS
Powdery scab resistance.


Session: Poster-41
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:189


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