| Abstract Detail
Breeding and Genetics Driscoll, Jarred [1], Douches, David [1], Hammerschmidt, Ray [2]. In-Vitro Screening Protocol for Resistance to Common Potato Scab. COMMON potato scab, caused by Streptomyces scabies, may be found worldwide wherever potatoes are grown. The disease incites cork-like lesions forming surface, raised, or pitted lesions on the dermal layer of the potato tuber. Since the marketplace for potatoes is quality driven, the presence of scab lesions, especially those which are pitted, significantly lessens the marketability for both tablestock and processing varieties. Traditional means for surveying scab resistant varieties include replicated field trials in soils with high concentrations of S. scabies and compare years of data, even though disease levels may vary from year to year due to environmental interactions. In this study, we used four protocols to assess disease reaction on a set of potato lines with known reaction to S. scabies. The thaxtomin A study was conducted where a known concentration of the phytotoxin was placed on potato tuber discs. There appears to be a hypersensitive response based on preliminary thin layer chromatography investigations. A spore suspension was use to inoculate potato tuber discs. This produced a general darkening of tuber tissue with no discrimination for resistance. A spore suspension was used to inoculate etiolated tuber sprouts. There was no response to this treatment. Currently, a green house study is underway. Streptomyces scabies was grown on vermiculite and this was used as an inoculum source in a sand and soil mixture. Obtaining a protocol that is both efficient and effective at discerning scab resistant varieties would be helpful for potato breeding programs in their early generation selection process, as well as limiting the amount of environmental influences.
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1 - Michigan State University, Department of Crop and Soil Sciences, 499B Plan and Soil Sciences Building, East Lansing, Michigan, 48824, United States
2 - Michigan State University, Plant Pathology, 35 Plant Biology Building, Wilson Road, East Lansing, MI, 48824, United States
Keywords:
none specified
Session: Poster-42
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:183 |
