| Abstract Detail
Conference Wide Gavrilenko, Tatjana [1], Antonova, Olga [2], Shvachko, Natalia [2], Kostina, Ludmila [2]. Diversity of nuclear, chloroplast and mitochondrial DNA in potato cultivars and Chilean landraces (Solanum tuberosum ssp. tuberosum). POLYMORPHISM of 10 nuclear SSR loci, 2 chloroplast (cp-) and 2 mitochondrial (mt-) loci was analyzed by PCR to study diversity of 20 Chilean landraces and of 98 potato cutivars from different countries. 10 SSRs (Kawchuk et al., 1996; Provan et al., 1996, Veilleux et al.,1995) were used to study levels of nuclear SSR polymorphism. To evaluate cpDNA polymorphism, cpSSR primers specific for NTCP9 locus (Provan et al., 1999) and ALC_1/ALC_3 primer pairs specific for cp- region atpE (Loessl et al., 2000) were used. To study mtDNA diversity primers specific for mt-regions rps10, atp6, cob (Loessl et al., 2000) were used. A set of specific primers from Loessl et al. (2000) was also used to detect three mt-types (α, β, γ) and two cp-types (T, W). Cp- and mt-specific primers showed polymorphism in both landraces and in cultivars. The combining of variants from two polymorphic cp-loci and two mt-loci generated eight haplotypes (I-VIII) with the most frequent haplotypes I (T/β) detected in 63% of cultivars and in 80% of landraces. Rare haplotypes (IV-VIII) were detected only among cultivars, at frequencies 1-2%. Of the 8 haplotypes detected, all were present in cultivars while only three haplotypes (I–III) were observed in landraces. The identification of landraces and cultivars by nuclear SSRs subdivided them into 5 clusters. Chilean landraces having haplotype I were quite homogeneous and separated into a distinct group. The results obtained indicate much lower levels of nuclear and cytoplasmic diversity in Chilean landraces. Higher levels of diversity observed in cultivars could reflect an introgression of wild and cultivated germplasm into the pedigrees of modern potatoes.
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1 - Vavilov Institute of Plant Industry, B.Morskaya, 42/44, St.-Petersburg, 190000, Russia
2 -
Keywords:
PCR markers
Potato
diversity.
Session: Poster-107
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:169 |
