| Abstract Detail
Conference Wide Ginzberg, Idit [1], Krits, Pini [1], Fogelman, Edna [1]. Regulation of Glycoalkaloids in Potato. GLYCOALKALOIDS (GA) are secondary metabolites characterized by a bitter taste and known to be toxic when consumed in large quantities. The two major GA present in potato (Solanum tuberosum) cultivars are α-chaconine and α-solanine that exhibit strong lytic properties and inhibit acetyl choline-esterase activity. Because of their toxic effect, commercial cultivars contain low level of GA, and their content in the edible tuber should not exceed 20mg/100gr FW. However, several factors associated with growth, harvest and post-harvest treatments, such as light exposure and wounding, might lead to an increment in tuber GA content. Therefore, it is desirable to develop new improved potato genotype that exhibit high GA level in leaves to protect the crop against insects and fungal diseases, and none in the edible tubers. The GA biosynthesis is derived from the isoprenoid pathway, however only sketchy details are available on GA specific genes. To understand the regulation of GA biosynthesis, the expression level of genes involved in the biosynthesis of sterol derivatives was monitored, by sqRT-PCR, in leaves and tuber skin of potato genotypes that exhibit various levels of GA content. Results indicated of correlation between high GA content and increased expression level of HMGR1, squalene synthase, and solanidine glucosyltransferase genes. Microarray and 2-D gel studies are currently under way to isolate GA specific genes.
Log in to add this item to your schedule
1 - The Volcani Center, Vegetable Research, P O Box 6, Bet Dagan, 50250, Israel
Keywords:
glycoalkaloids
isoprenoid pathway.
Session: Poster-106
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:165 |
