| Abstract Detail
Conference Wide Finkers, Richard [1], van Heusden, Adriaan W. [1], van Kan, Jan [2], Lindhout, Pim [1]. Identification of QTLs for Botrytis cinerea resistance in S. habrochaites LYC4. BOTRYTIS cinerea Pers:Fr (teleomorph: Botryotina fuckeliana (de Bary) Whetzel) is a necrotrophic pathogenic fungus with an exceptionally wide host range of at least 235 species. Modern hybrid tomato (Solanum lycopersicum) cultivars are susceptible to B. cinerea although there are cultivars with some quantitative resistance. In a screening Solanum habrochaites LYC4 showed a good level of resistance. To study the genetics behind this resistance, an F2 population (n=174) of the cross between a susceptible genotype S. lycopersicum cv. Moneymaker and S. habrochaites LYC4 was developed. Initially, two QTLs for resistance to B. cinerea were identified (QTL1 and QTL2). During the confirmation of these two QTLs in segregating BC2S1 families, a third QTL (QTL3) was identified from which the effect could only be observed in absence of QTL2. A set of QTL specific CAPS makers was developed as a tool for marker assisted selection (MAS). Using this set of markers, one BC2S1 line was selected which was homozygous for both QTL 1 and QTL3. BC2S2 offspring of this line was tested for resistance to B. cinerea in a greenhouse trail. In the selected BC2S2 offspring only 15% of the infections were successfully established (disease incidence). This is significantly less than the susceptible control S. lycopersicum (disease incidence 70%); a commercial cultivar known as fairly resistant still had a disease incidence of 50%. This result shows that the QTLs from S. habrochaites LYC4 offer good perspectives for breeding B. cinerea resistant tomato cultivars.
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Department of Plant Breeding
Laboratory of Phytopathology
1 - Wageningen University, Laboratory of Plant Breeding, P.O Box 386, Wageningen, 6700AJ, The Netherlands
2 - Wageningen University, Phytopathology, P.O. Box 8025, Wageningen, 6700 EE, The Netherlands
Keywords:
B. cinerea
QTL
Disease resistance
S. habrochaites
gray mold
CAPS
AFLP.
Session: Poster-104
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:155 |
