| Abstract Detail
Conference Wide Orsi, Cintia Hotta [1], Tanksley, Steve [2]. Progress Towards the Cloning of sw4.1., the Major Seed Size Gene, in Tomato: From Fine Mapping to BAC Sequence Analysis. SEED size is a life-history trait whose significance is emphasized by its ecological, agronomic and evolutionary importance. In nature, seed size is associated with fitness, playing a major role in seed size-number trade-off. Seed size increase was also one of the consequences of the domestication process for most of the crops, including seed crops. Despite the obvious importance of seed size, the genetic and molecular bases for variation in seed size in nature is still largely unknown. Previous studies in tomato have identified a QTL, sw4.1., which accounts for a large portion of the seed size difference between wild and cultivated tomatoes. Nearly isogenic lines have been created for the fine mapping of this seed size QTL. A subsequent screening of 10,000 F2 individuals from a cross between the NILs has allowed us to narrow down the seed-size controlling region to 30 kb within a single BAC clone. A combination of association genetics and transformation/complementation experiments are being used to pinpoint the specific gene involved and the nature of the genetic changes in this gene (or its promoter) which are causally related to natural variation in seed size.
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1 - Cornell University, Plant Breeding and Genetics, 240 Emerson Hall - Cornell University, Ithaca, NY, 14853, USA
2 - Cornell University, Plant Breeding and Genetics, 248 Emerson Hall - Cornell University, Ithaca, NY, 14853, USA
Keywords:
seed
size
QTL
fine mapping
Domestication
candidate genes
Gene mapping.
Session: Poster-103
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:153 |
