| Abstract Detail
Conference Wide Alvarez, Adriana [1], Broglia, Vivianne [2], Alberti D´Amato, Anahi [1], Tjallingii, Freddy [3], Dicke, Marcel [3], Vosman, Ben [1]. Local and systemic responses of Solanum stoloniferum to infestation by aphids: induce resistance and gene expression. WILD tuber-bearing potato Solanum stoloniferum (Schlechtd) possesses a high constitutive resistance towards the green-peach aphid, Myzus persicae (Sulzer); this was evident from our previous aphid probing behavior, settling behavior, nymph mortality and colony development studies. By electrical penetration graph (EPG) monitoring we could localize a resistance factor at the epidermal/mesophyll level, disturbing the mechanical activities of the aphid stylet. On the other hand S. stoloniferum showed low or moderated level of resistance towards Macrosiphum euphorbiae. Gene expression studies were carried out in S. stoloniferum after infestation with M. persicae or with M. euphorbiae, using a dedicated cDNA microarray containing 3800 defense-related potato genes.. We analyze gene expression at the local and systemic level after attack by each of the aphids. For each plant-aphid combination the following binary comparisons were made: (1) pre-infested local leaf vs. non-infested systemic leaf of the same plant individual, and (2) pre-infested leaf vs. non-infested leaf (control plant) of different plant individuals, and using leaves at similar position as the systemic and local leaves in the previous test. Differential expression was observed for a specific set of genes. Aphid settling behavior was tested with M. persicae on S. stoloniferum after pre-infestation of the plants with M. persicae or M. euphorbiae for 96 hours. The test was performed to study induced resistance on the previously infested leaf (local responses), and on a higher non-infested leaf (systemic response). The results will be discussed during the meeting.
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1 - Plant Research International, Bioidversity and Breeding, P.O.Box 16, Wageningen, 6700 AA, Netherlands
2 - Universidad Nacional de Salta, Facultad de Ciencias Naturales, Buenos Aires 177, Salta, 4400, Argentina
3 - Wageningen University, Laboratory of Entomology, P.O. Box 8031, Wageningen, 6700 EH, Netherlands
Keywords:
none specified
Session: Poster-102
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:152 |
