| Abstract Detail
Conference Wide Wang, M. [1], van den Berg, R.G. [2], van der Linden, C.G. [1], Vosman, Ben [1]. The potential of NBS profiling for potato systematics. POTATO (Solanum tuberosum L.) is a crop with a large secondary genepool, which contains many important resistance traits that could be exploited in breeding programs. Pyramiding of several resistance genes from different sources is a strategy that decreases the chances of the pathogen breaking the resistance. Systematic relationships might be used to select material that has the highest chance of containing different resistance genes for a particular disease. Recently, a novel molecular technique, called NBS profiling, was developed by van der Linden et al. (2004). This gene-targeting technique produces multiple markers in resistance genes and their analogs (RGAs) and might therefore be even more suitable for selecting such materials than techniques like AFLP. In this study we have compared NBS profiling with AFLP for their potential in reconstructing phylogenies in tuber-bearing wild Solanum species. We have used a set of over 100 genebank accessions, encompassing more than 50 species. Sequencing and annotation of the bands from the NBS profiles showed that the average RGA content is 73%, indicating that indeed NBS profiles largely contain markers in R-genes and RGAs. Parsimony and neighbour joining analysis produce highly similar trees for NBS profiling and AFLP markers, indicating that NBS profiling is powerful and comparable to AFLP in phylogeny reconstruction. The fact that the trees were so similar even though NBS profiling targets a specific subset of genes might be related to the random distribution of NBS-LLR resistance genes in the genome. Reference: Van der Linden CG, Wouters DCAE, Mihalka V, Kochieva EZ,Smulders MJM & Vosman B (2004) Efficient targeting of plant disease resistance loci using NBS profiling. Theor. Appl. Genet.109:384–393.
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1 - Plant Research International, Wageningen, 6700 AA Wageningen, The Netherlands
2 - Wageningen University, Biosystematics Group, Gen. Foulkesweg 37, Wageningen, 6703 BL Wageningen, The Netherlands
Keywords:
AFLP
NBS profiling
Solanaceae systematics.
Session: Poster-101
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:151 |
