| Abstract Detail
Breeding and Genetics Leite, Daniela [1], Anthonisen, Denilson [1], Barbieri, Rosa Lia [1]. Estimation of genetic divergence in pepper (Capsicum baccatum var. pendulum) revealed by RAPD markers. THE pepper (Capsicum spp.) culture occurs in almost all Brazilian regions. It is one of the best examples of familiar agriculture and of the integration of small growers and industry. The market is demanding for new cultivars with higher productivity and with different sizes, shapes, colors, flavors and pungency of the fruits. In order to attend these demands, our institution develops works in characterization and preservation of all the available genetic variability associated to genetic breeding. The objective of this work was to evaluate molecularly, trough the RAPD analysis (Random Amplified Polimorphic DNA), the existing genetic diversity in a group of populations cultivated in the South Region of Brazil. It was analyzed four primers, previously selected, in DNAs from a total of 81 individual plants from 9 populations. The plants were genotyped through the products visualized in agarose gels and a matrix of presence/absence of bands was used in the analyses of genetic similarity and grouping (coefficient of Jaccard). The four primers generated 48 bands, being 37 polimorphic. The number of fragments per primer varied from 6 (primer OPAB 8) to 16 (primer OPA 2) with an average of 12 bands per primer. The grouping analysis allowed the separation of populations in 13 groups, with in general, each population belonging to one group or to a very close groups, with an average of 65% of similarity.
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1 - Embrapa Clima Temperado, Molecular Biology Laboratory, Br 392, km 78, Caixa Postal 403, Pelotas, RS, 96001-970, Brazil
Keywords:
Germplasm
RAPD
genetic variability.
Session: Poster-44
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:150 |
