| Abstract Detail
Conference Wide Laursen Høgh, Annabeth [1], Emmersen, Jeppe [1], Nielsen, Kåre L. [1]. Transcript profiling of potato tuber development. SOLANUM tuberosum (potato) is the fourth major crop worldwide and used for food, feed and biotechnological applications. To fully realize the biosynthetic potential for production of starch, protein and metabolites, we conducted an extensive quantitative profiling of the expressed genes of 5 different developmental stages of the potato tuber (cv. Kuras) by Serial Analysis of Gene Expression (SAGE): 8 week old plants (~1 cm soil grown mini tuber), tubers harvested at the end of flowering (mature tuber), at harvest, from tubers stored for 30 days (dormant), and from tuber tissue just below the shoot of a sprouting tuber. Three developmental stages were analyzed with traditional LongSAGE encompassing approximately 50,000 tags, whereas harvest and dormant were analyzed using the novel DeepSAGE approach (see separate poster presentation) to a depth of 143,411 and 122,100 tags, respectively. Pair wise comparisons of SAGE libraries were conducted, and significantly up or down regulated genes was identified using the Z-test at 98% confidence. In general extensive changes were observed, indicating that the tuber is not merely a storage organ, but indeed a metabolically active and dynamically regulated plant organ. Interesting observations with regard to transcripts encoding storage proteins, proteases and their inhibitors and metallothioneins will be discussed.
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1 - Aalborg University, Department of Biotechnology, Chemistry and Environmental Engineering, Sohngaardsholmsvej 49, Aalborg, 9000, Denmark
Keywords:
development
Potato
tuber
gene expression.
Session: Poster-100
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:149 |
