| Abstract Detail
Breeding and Genetics Cooper, Susannah [1], Douches, David [1], Grafius, Edward [2]. Insecticidal activity of transgenic potatoes (Solanum tuberosum L.) expressing avidin against Colorado potato beetle larvae, Leptinotarsa decemlineata (Say). THE Colorado potato beetle, Leptinotarsa decemlineata (Say), is the most destructive insect pest of potato, Solanum tuberosum (L.) in eastern North America. Biotin is an essential co-enzyme required for all organisms, including insects. Avidin binds to biotin, therefore limiting its availability during insect growth and development. Without this co-enzyme, an insect’s growth is severely stunted, eventually leading to death. We have expressed avidin in two potato lines: MSE149-5Y, a susceptible potato line, and ND5873-15, a high glycoalkaloid line. The expression levels of avidin in the transgenic MSE149-5Y and ND5873-15 was determined to be 108.6 ± 0.4µg /mg and 108.2 ± 0.9µg/mg, respectively. Detached leaf bioassays were performed on transgenic and non-transgenic lines of MSE149-5Y and ND5873-15 using Colorado potato beetle first and third stage larvae. First stage larvae survivorship was significantly less for larvae feeding transgenic MSE149-5Y and ND5873-15 lines compared to the non-transgenic lines, but third stage larvae survivorship did not significantly differ for larvae feeding on transgenic MSE149-5Y and ND5873-15 lines compared to larvae feeding on non-transgenic lines. The growth of first stage larvae was significantly stunted for larvae feeding on the transgenic MSE149-5Y and ND5873-15 lines compared to the non-transgenic lines. Subsequently, we placed neonates on non-transgenic and transgenic MSE149-5Y and ND5873-15 lines and monitored growth and development of insects. Avidin expressing potato plants appeared to delay development and resulted in significantly less emerging adults. Potatoes expressing avidin may have value in managing insect pests.
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1 - Michigan State University, Department of Crop and Soil Sciences, 499B Plan and Soil Sciences Building, East Lansing, Michigan, 48824, United States
2 - Michigan State University, Department of Entomology, 442 Natural Science, East Lansing, Michigan, 48824, United States
Keywords:
Host plant resistance.
Session: PAA09-7
Location: Hall of Ideas Room G/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 3:30 PM
Abstract ID:144 |
