| Abstract Detail
Tomato sequencing - Morning Lee, Sanghyeob [1], Cho, Sung-Hwan [1], Hur, Chul-Goo [1], Park, Hong-Seok [2], Koo, Dal-Hoe [1], Bang, Jae-Wook [3], Choi, Doil [1]. Sequencing of Tomato Chromosome 2. THE goal of SOL project is to sequence the approximately 220 Mb of euchromatin that may be expected to contain the majority of coding sequences, rather than the 950 Mb of tomato full genome. The Korea team is aiming to complete sequencing of ca. 26 Mb euchromatin in chromosome 2 using a minimal tiling path of BAC clones. From the initial sequencing stage, 39 BAC clones have been selected as “seed BAC” by overgo probe hybridization and FISH analysis. The 67% (34 BAC clones) of seed BACs can be extended for further sequencing. The average overlapping length between seed BAC and next BAC is 18 kb. Currently we are focusing on (1) further BAC extension using BES (BAC End Sequence) database (2) BAC filter hybridization when extendible BAC is not available using BES. The current status of sequencing progress will be presented.
Log in to add this item to your schedule
1 - Korea Research Institute of Bioscience and Biotechnology, Plant Genomics Lab, P.O. Box. 115, Taejon, 305-600, South Korea
2 - Korea Research Institute of Bioscience and Biotechnology, Genomic Research Center, P.O. Box. 115, Taejon, 305-600, South Korea
3 - Chungnam National University, Biology, Chungnam National University, Taejon, 305-764, South Korea
Keywords:
tomato
FISH
sequencing
.
Session: SAT04-3
Location: Hall of Ideas Room E/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 8:45 AM
Abstract ID:128 |
