| Abstract Detail
Tomato sequencing - Morning Asamizu, Erika [1], Kaneko, Takakazu [1], Sato, Shusei [1], Nakamura, Yasukazu [1], Ono, Akiko [1], Watanabe, Akiko [1], Tabata, Satoshi [1]. Tomato genome sequencing at Kazusa DNA Research Institute. AS a member of the Solanaceae Genomics Network (SGN) International Tomato Sequencing Project, we launched on sequencing of the chromosome 8. Our mission is to sequence the euchromatic region of the chromosome, the estimated size of which is 17 megabases (Mb). BAC-by-BAC strategy is adopted for sequencing. We initially received BAC clone candidates anchored to overgo probes corresponding to 33 genetic markers on chromosome 8. For confirmation of the BAC clones, we performed PCR amplification with primers designed from the marker sequence using the BAC DNA as template. Twenty-five BAC clones were validated by sequencing the amplified fragment and subjected to shotgun sequencing. As of March 2006, we finished 18 BACs to Phase 3 (total length 2,274,432 bases), one BAC to Phase 2 and another to Phase 1. Five BACs are being sequenced. First round of BAC extension was performed for finished 14 BACs. We found that 12 of them could be extended with the maximum overlap of 20 kb. To select additional BACs for sequencing, we are planning to make a 3D DNA pool of LE-HBa, SL-MboI and SL-EcoRI BAC libraries, which enables PCR screening. We will present the latest status of our project.
Log in to add this item to your schedule
1 - Kazusa DNA Research Institute, Dept. Plant Gene Research, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan
Keywords:
genome sequencing.
Session: SAT04-9
Location: Hall of Ideas Room E/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 10:15 AM
Abstract ID:124 |
