| Abstract Detail
Thursday Morning Contributed Talks Guzman-Diaz, Felix [1], Moore Fellman, Shanna [2], de Vicente, Carmen [3], Jahn, Molly [2]. Development of a molecular tool to allow worldwide comparison of Capsicum germplasm. CAPSICUM germplasm represents a valuable source of diversity that remains under-investigated. Molecular markers, if carefully selected, could be a useful characteristic to assess genetic diversity of Capsicum germplasm worldwide and could contribute to the evaluation of actual diversity conserved in both ex situ and in situ collections. This project was designed to assess the utility of a collection of publicly available single sequence repeat (SSR) markers for distinguishing a diverse panel of Capsicum germplasm. A selection of Capsicum germplasm representing 12 species was utilized in conjunction with a panel of 18 SSR markers. A total of 170 alleles were detected at the 18 SSR loci. Polymorphism information content (PIC) values ranged between 0.33 (Nagy 20) to 0.93 (Bd 41), with an average of 0.73 per marker. Genetic relationships among Capsicum genotypes as determined by Multiple Correspondence Analyses (MCA) and a dendrogram from the UPGMA analyses of Dice genetic similarity grouped the genotypes into clusters having varying degrees of similarity. These clusters correspond mainly to C. annuum, C. chinense, C. chinense-C. frutescens and C. baccatum. Genotypes belonging to C. chacoense, C. pubescens, C. minutifolium, C. cardenasii and C. flexuosum also clustered according to their taxonomic designation. Two genotypes of C. rhomboideum, a species considered basal to the genus, showed the lowest similarity to all other genotypes. A number of SSRs have been identified which can be used to differentiate among Capsicum species. These results could be useful in genotype identification, monitoring purity and Capsicum genetic resources conservation.
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1 - Universidad del Valle, Departamento de Biología, Apartado Aereo 25360, Cali, Valle, , Colombia
2 - Cornell University, Department of Plant Breeding and Genetics, Ithaca, NY, 14853, USA
3 - International Plant Genetic Resources Institute (IPGRI), c/o CIAT, Apartado Aereo 6713, Cali, Valle, , Colombia
Keywords:
Capsicum
conservation
Microsatellites.
Session: SOL06-8
Location: Ballroom AB/Monona Terrace
Date: Thursday, July 27th, 2006
Time: 10:50 AM
Abstract ID:122 |
