| Abstract Detail
Conference Wide Moloney, Claire [1], Griffin, Denis [1], Bradshaw, John [2], Bryan, Glenn [2], Milbourne, Dan [1]. Developing MAS for the major heritable component of the H3-based resistance to Globodera pallida pathotype Pa2/3 in potato. THE potato cyst nematode Globodera pallida pathotype Pa2/3 is one of the most significant soil-borne pests in the UK, and developing cultivars expressing high levels of resistance to this pathogen is a major goal of potato breeding programmes targeting the UK growers market. One excellent source of resistance to this nematode species/pathotype is the polygenic H3 source of resistance originally derived from Solanum tuberosum ssp andigena accession CPC2802. This resistance source is actively being used in both the SCRI and Oak Park breeding programmes, and a large effect QTL, named Gpa4, has now been mapped to chromosome IV in advanced tetraploid breeding clones called 12601ab1 (from SCRI) and 1992/31 (from Oak Park), which have both been developed from CPC2802 by different routes. The QTL is in the duplex dosage state in 12601ab1, and the simplex dosage state in 1992/31. Developing diagnostic markers for the genes underlying the resistance phenotype is a high priority at both research centres. We are currently identifying SNP markers from the Gpa4 interval by sequencing genetically-anchored BAC clones from this region, and testing these for their diagnostic value for the Gpa4 QTL in a panel of genotypes including both 12601ab1, 1992/31 and other genotypes which are either derived from CPC2802 or not. Confirmation of the presence in Gpa4 in 12601ab1 and 1992/31, and knowledge of the different dosage states of the QTL in these breeding lines is likely to be of benefit in identifying markers truly diagnostic of the Gpa4 QTL using this approach.
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1 - Teagasc Crops Research Centre, Oak Park, Carlow, , Republic of Ireland
2 - Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK
Keywords:
Potato.
Session: Poster-88
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:109 |
