| Abstract Detail
Breeding and Genetics Carputo, Domenico [1], Barone, Amalia [1], Caruso, Immacolata [1], Iorizzo, Massimo [1], Zoina, Astolfo [2], Frusciante, Luigi [3]. Fertile Solanum commersonii-S. tuberosum sexual hybrids as source of resistance to Ralstonia solanacearum. AS part of a breeding program aimed at transferring useful resistances from 2x(1EBN) S. commersonii to S. tuberosum through sexual hybridization, we have developed (aneuploid)pentaploid hybrids displaying noteworthy characteristics. The objectives of the present investigation were to characterize their resistance to bacterial wilt caused by Ralstonia solanacearum and to efficiently select best fertile genotypes. Hybrids showed a high variable response after artificial inoculations, segregating from resistant to susceptible reactions. Null effect hypothesis about genotypes and time after inoculation on disease index suggested that the two factors considered and their interaction were significant sources of variation. Principal Component Analysis was performed to draw out differences among hybrids in term of disease index. The cDNA-AFLP-TP technique was used to evidence putative genes differentially expressed in resistant S. commersonii and susceptible S. tuberosum. cDNA-AFLPs fragments expressed only in the resistant genotype are being sequenced in order to define if they may represent genes induced by infection. Male and female fertility of hybrids was analyzed in reciprocal crosses with S. tuberosum. Although fertility was reduced compared to conventional 4x x 4x crosses, sizable progenies with practical utility were produced. Results provided evidence that 5x x 4x crosses performed better than 4x x 5x crosses, thus suggesting that the male gametophyte is more sensitive to aneuploidy than is the female. None of the fertility parameter analyzed was significantly correlated with the somatic chromosome number of the (aneuploid)pentaploid hybrids analyzed.
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1 - University of Naples Federico II, Department of Soil, Plant and environmental Sciences, Via Università 100, Portici (NA), Italy, 80055
2 - University of Naples \"Federico II\", Fruit Crops, Botany and Plant Pathology, Via Università 100, Portici, 80055, Italy
3 - University Federico II of Naples, Soil, Plant and Environmental Sciences, , Italy
Keywords:
aneuploidy
Molecular markers
EBN.
Session: PAA08-12
Location: Hall of Ideas Room G/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 11:45 AM
Abstract ID:101 |
